Specialized Training Program

1. High Performance Liquid Chromatography Technique:

Acentric Biotech offers an outstanding platform for training on HPLC. The training is especially designed for those individuals who want to have an exposure to High Performance Liquid Chromatography technique. This training program provides theoretical and practical understanding of HPLC, its maintenance, method development and troubleshooting techniques.

[A] Beginner's Level:

• i. Introduction to Chromatographic Technique.
• ii. Introduction to Liquid Chromatography and it's types.
• iii. Fundamentals of HPLC Technique.
• iv. Hands On training on Instrumentation- Parts & it's working.

[B] Intermediate Level:

• i. Introduction to HPLC & it's components.
• ii. Types of Pumps, Columns, Detectors and their uses./li>
• iii. Types of Mobile Phase and Stationary Phase.
• iv. Explanation of Titrimetric Calculations.
• v. Preparation of test solutions and Mobile Phase.
• vi. Development of method and optimizing various chromatographic parameters.
• vii. Identification, Quantification, Impurity purity analysis of compounds.
• viii. Introduction to Data acquisition software, Integration and Report Generation.
• ix. Understanding specific applications, preventive care, maintenance and trouble shooting.

Program Fees Details:

Level	Duration	Course Fees	Seats Availability	Eligibility
Beginner's	7 Days	Rs. 5,500/-	20	Visit Benefits and Eligibility page
Intermediate	12 Days	Rs 9,500/-	10

2. Stem Cell & Tissue Engineering:

Acentric Biotech offers an outstanding platform for training on Stem Cell & Tissue Engineering. The training is especially designed for those individuals who want to have an exposure to Stem Cell & Tissue Engineering technique. This training program provides theoretical and practical understanding of Stem Cells, their isolation, differentitation methods and their maintenance.

[A] Beginner's Level:

• i. Introduction to stem cell and Characteristics of stem cells, Introduction to tissue engineering - cells as therapeutic agents and Types of stem cell – Sources and potency.
• ii. Standard Operating Procedures of Stem Cell Intruments.
• iii. Preparation of chemicals, reagents & buffers.
• iv. Isolation of human mononucleated cells from peripheral blood.
• v. Cell count by Hemocytometer.
• vi. Culturing and maintenance of isolated pluripotent and multipotent human peripheral blood stem cells.

[B] Intermediate Level:

• i. Introduction to stem cell and Characteristics of stem cells, Introduction to tissue engineering - cells as therapeutic agents and Types of stem cell – Sources and potency.
• ii. Standard Operating Procedures of Stem Cell Intruments.
• iii. Preparation of chemicals, reagents & buffers.
• iv. To isolate dental pulp mesenchymal stem cells (MSCs) from non-infected human permanent teeth.
• v. Cell count by Hemocytometer to count dental pulp mesenchymal stem cells (MSCs).
• vi. Culturing and maintenance of isolated mesenchymal stem cells (MSCs).

[C] Advance Level:

• i. Introduction to stem cell and Characteristics of stem cells, Introduction to tissue engineering - cells as therapeutic agents and Types of stem cell – Sources and potency.
• ii. Standard Operating Procedures of Stem Cell Intruments.
• iii. Preparation of chemicals, reagents & buffers.
• iv. Isolation of human mononucleated cells from peripheral blood.
• v. Cell count by Hemocytometer.
• vi. Culturing and maintenance of isolated pluripotent and multipotent human peripheral blood stem cells.
• vii. Characterization of pluripotency and multipotency human peripheral blood stem cells by Phase contrast microscope.
• viii. Cytochemical staining of Osteoblasts derive from hPBSCs.
• ix. Chondrogenic differentiation of stem cells using Alcian blue staining.
• x. To isolate dental pulp mesenchymal stem cells (MSCs) from non-infected human permanent teeth.
• xi. Cell count by Hemocytometer to count dental pulp mesenchymal stem cells (MSCs).
• xii. Culturing and maintenance of isolated mesenchymal stem cells (MSCs).
• xiii. Adipogenic differentiation of mesenchymal stem cells (MSCs) using Oil red O staining.

Program Fees Details:

Level	Duration	Course Fees	Seats Availability	Eligibility
Beginner's	10 Days	Rs. 8,000/-	10	Visit Benefits and Eligibility page
Intermediate	15 Days	Rs. 12,000/-	10
Advance	45 Days	Rs. 27,000/-	5

3. In Vitro Pharmacology:

This course introduces various aspects of the drug discovery process including target identification, medicinal chemistry and in vitro drug screening methods. Students will gain a broad practical knowledge of drug discovery research base that will allow them to prepare for a career in pharmaceutical research or basic medical science with implications for novel therapeutics.

[A] Beginner's Level:

• i. Introduction to In Vitro Pharmacology and Its Role in the Drug Discovery and Development Cycles.
• ii. Standard Operating Procedures of In Vitro Pharmacology Instruments.
• iii. Collection & Preparation of medicinal plants.
• iv. In-vitro Anti-diabetic activity assay by alpha-amylase.
• v. In-vitro Anti-diabetic activity assay by alpha-Glucosidase Inhibition assay.
• vi. Anti-oxidant activity by Ferric reducing assay.
• vii. Anti-oxidant activity by DPPH method.

[B] Intermediate Level:

• i. Introduction to In Vitro Pharmacology and Its Role in the Drug Discovery and Development Cycles.
• ii. Standard Operating Procedures of In Vitro Pharmacology Instruments.
• iii. Collection & Preparation of medicinal plants.
• iv.Preparation of buffers for suspension.
• v. Preparation of RBC suspension by Membrane stabilization.
• vi. Anti-inflammatory activity by Inhibition of albumin denaturation.
• vii. Anti-inflammatory activity by proteinase inhibitory assay.
• viii. Haemolytic activity by Heat induction.
• ix. Haemolytic activity by Hypo tonicity induction

Program Fees Details:

Level	Duration	Course Fees	Seats Availability	Eligibility
Beginner's	10 Days	Rs. 8,000/-	10	Visit Benefits and Eligibility page
Intermediate	15 Days	Rs. 15,000/-	5

4. Cryobiology:

This course introduces various aspects of the drug discovery process including target identification, medicinal chemistry and in vitro drug screening methods. Students will gain a broad practical knowledge of drug discovery research base that will allow them to prepare for a career in pharmaceutical research or basic medical science with implications for novel therapeutics.

[A] Beginner's Level:

• i. Introduction to Cryobiology and Understanding it's role in the preservation of bacterial cell isolate .
• ii. Standard Operating Procedures of Cryobiology Instruments.
• iii. Isolation of pathogenic bacterial strain from wastewater.
• iv. Primary identification and staining of bacterial strain.
• v. Counting of Bacterial Colony forming units using Colony Counter and Multiplication of bacterial strain by broth preparation.
• vi. Cryopreservation of bacterial strain.
• vii. Evaluation of bacterial cells survival.

[B] Intermediate Level:

• i. Introduction to Cryobiology and Understanding it's role in the preservation of fungal cell isolate.
• ii. Standard Operating Procedures of Cryobiology Instruments.
• iii. Isolation of pathogenic fungal strain from .
• iv. Morphological identification and staining of fungal strain.
• v. Multiplication of fungal strain by broth preparation.
• vi. Cryopreservation of fungal strain.
• vii. Estimation of viability and activity of fungal cells.
• viii. To learn freeze drying technique of fungal cell cultures.
• ix. Evaluation of fungal cells survival.

Program Fees Details:

Level	Duration	Course Fees	Seats Availability	Eligibility
Beginner's	7 Days	Rs. 5,000/-	10	Visit Benefits and Eligibility page
Intermediate	10 Days	Rs. 7,500/-	5

[B] Advance Level:

• i. Introduction to Cryobiology and Understanding it's role in the preservation of fungal cell isolate.
• ii. Standard Operating Procedures of Cryobiology Instruments.
• vi. Preparation of various vitrifications solutions for plant cell culture.
• vii. Extraction and cryopreservation of plant cell culture using vitrification technique.
• viii. Preparation of solution for encapsulation dehydration technique.
• ix. Cryopreservation of embryonic axes by recombinant bacterial cultures.
• x. Extraction, cryopreservation and viability assessment of pollen.

Program Time & Availability:

Timings: 09:00 AM to 01:00 PM & 02:00 PM to 06:00 PM

Days: Monday to Saturday (Note:Second & Fourth Saturday & Sunday training is closed)

Program Benefits & Opportunities:

Certified with ISO 9001:2015 and MSME, Govt. of India

India’s first customizable training as per your need

Individual hands-on session. No batch system

Practical learning along with theoretical session

Individual attention by guide in every experiments

After completion of certificate,get an Industrial training certificates which will be valid in India & Worldwide

Free authentication of Certificate for next 25 years

Accomodation such as PG's and Room facility is available near campus under 4000 per month (cost varies)

Program Eligibility: Visit Benefits and Eligibility page

How To Apply:

In order to join training program, all the prospective participants will have to fill the online application by clicking on "Apply Online" button. Once the online form is submitted, pay registration fee of Rs 600/- via Paytm/Google Pay/UPI/BHIM/Phone Pe/NEFT and receive the seat confirmation letter on mail.